ConfocalConfocal Microscopes – Leuven

confocal_leuven_Biorad_cleaned

Biorad Radiance 2100

Location KU Leuven, campus Gasthuisberg, Building O&N4
7th floor, Room 07.107
Type Nikon upright
Light Source Laser; 405nm (blue laser diode), 488nm (40mW Argon laser), 543nm (1,5mW HeNe laser) & 667nm (HeNe laser)
Properties Fast acquisition, High resolution
Objective lenses Plan Fluor 10X (0,30 dry), Plan Fluor 20X (0,75 dry), Plan Apo 40X (1,00 oil), Plan Fluor 60X (1,00 water) & Plan Apo 60X (1,40 oil)
Filters Hg-bulb DAPI (340-380/400/435-485), FITC (465-495/505/515-555) & TRITC (510-560/575/590 LP)
Filters laser Block filters: HQ 420, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600 LP and Emission filters: HQ 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610 SP & 620 LP & 590/70

Nikon C2

Nikon C2 Eclipse Ni-E

Location KU Leuven, campus Gasthuisberg, Building O&N4
7th floor, Room 07.107
Type Nikon inverted
Light Source Laser; 405nm, 488nm, 561nm & 640nm
Properties Fast acquisition, high resolution, JOBS software for automatic aqcuisition possible, PFS system for zero drift
Objective lenses Plan Apo 10X (0,45 dry), Plan Apo VC 20X (0,75 dry), Plan Fluor 40X (1,30 oil), Apo 60X (1,40 oil)
Filters Hg-bulb DAPI, FITC & TRITC
Filters laser 425-475, 465-500, 500-550, 525-555, 500-530, 570-620, 553-618 & 663-738
Acknowledgements This device was acquired by the VIB Bio Imaging Core. Acknowledgement of the funding in resulting publication is required.

confocal_leuven_Nikon-A1R-Eclipse1_cleaned

Nikon A1R Eclipse Ti

Location KU Leuven, campus Gasthuisberg, Building O&N4
7th floor, Room 07.401
Type Nikon inverted
Light Source Laser; 405nm, 488nm, 561nm & 640nm
Properties Fast acquisition, high resolution, equipped with a spectral detector, live cell imaging (incubator with T and CO2 control), TIRF, JOBS software for automatic aqcuisition possible, PFS system for zero drift
Objective lenses Plan Apo 4X (0,20 dry), Plan Apo 10X (0,45 dry), Plan Apo VC 20X (0,75 dry), Plan Fluor 40X (1,30 oil), Apo 60X (1,40 oil) & Apo TIRF 100x (1,49 oil) DIC N2 WD 0,12
Filters Hg-bulb DAPI, FITC & TRITC
Filters laser 425-475, 465-500, 500-550, 515-550, 525-555, 500-530, 570-620, 560-618 & 663-738
Acknowledgements This device was acquired through a Hercules type 1 AKUL/09/037 grant to Wim Annaert. Acknowledgement of the funding in resulting publication is required.
confocal_leuven_Leica-SP51cleaned

Leica TCS SP5 II

Location KU Leuven, campus Gasthuisberg, Building O&N4
7th floor, Room 07.401
Type Leica upright
Light Source Laser; 458nm (Argon), 476nm (Argon), 488nm (Argon), 514nm (Argon), 543nm (HeNe 543) & 633nm (HeNe 633)
Properties high resolution, 2 HyD detectors (more sensitive, less background), adjustable filters
Objective lenses HCX PL Fluotar 10X (0,30 dry), HC PL Apo 20X (0,75 dry), HCX PL Fluotar 40X (0,75 dry) & HCX PL Apo 63X (1,40 oil)
Filters Hg-bulb Green, Red & Far-red
Filters laser Adjustable
confocal_leuvenSp-disk1_cleaned

Nikon Spinning disk

Location KU Leuven, campus Gasthuisberg, Building O&N4
7th floor, Room 07.401
Type Nikon upright
Light Source Laser; 405nm, 488nm, 561nm & 640nm + 405nm for FRAP
Properties spinning-disk confocal (physiology setup) for fast confocal imaging, 2 camera system, Mosaic unit & 405nm laser for FRAP
Objective lenses Fluor 20X (0,50 water DIC M/N2), Fluor 40X (0,80 water DIC M/N2) & Plan Apo 60X (1,40 oil WD 0,13) – more possible on request
Filters Hg-bulb DAPI (Ex 387/11nm), GFP (Ex 472/30nm – Em 520/35nm) & TRITC (Ex 543/22nm – Em 593/40nm)
Filters laser Quadrupole filter, blue 405 single – 450/50, green 488 single – 525/50, red 561 single – 595/50 & far-red 640 single – 700/75
Acknowledgements This device was aquired through a CLME grant from Minister Lieten to the VIB BioImaging Core. Acknowledgement of the funding in resulting publication is required.
confocal_leuven_Nikon-PV1_cleaned

Nikon Eclipse Ni-E

Location KU Leuven, campus Gasthuisberg, Building O&N4
7th floor, Room 07.439
Type Nikon upright
Light Source Laser; 405nm, 488nm, 561nm & 638nm
Properties High resolution, fast acquisition, physiology setup, 2 GaAsP detectors (more sensitive, less background)
Objective lenses Apo LWD 40x (1,15 WI lambdaS DIC N2 WD 0,59-0,61) & NIR Apo 60x (1,0 W DIC N2 WD 2,8) & Plan Apo 60x (1,40 oil OFN25 DIC N2 WD 0,13) – more possible on request
Filters Hg-bulb DAPI, FITC & TRITC
Filters laser 425-475, 465-500, 500-550, 525-555, 500-530, 570-620, 553-618 & 663-738
confocal_leuven_Zeiss_LSM5101_cleaned

Zeiss LSM 510

Location KU Leuven, campus Gasthuisberg, Building O&N4
6th floor, Room 06.107
Type Zeiss upright
Light Source Laser; 488nm, 543nm & 637nm
Properties high resolution, physiology setup
Objective lenses Achroplan 10x (0,30 W) Ph1, Plan-Apochromat 40x (1,30 oil) DIC (UV) VIS-IR, IR-Achroplan 40x (0,80 W), Plan-Apochromat 63x (1,0 W) VIS-IR, Plan-Apochromat 63x (1,4 oil) & IR-Achroplan 63x (0,90 W)
Filters Hg-bulb Green, Red & Far-red
Filters laser NT 80/20, HFT (UV/488/543/633, KP 700/488, KP 700/543, 458/514, 458, KP 650), BG 39, NFT (490, 515, 545, 635 VIS & KP 545), BP (390-465 IR, 435-485 IR, 480-520 IR, 500-530 IR, 510/20 IR, 500-550 IR, 535-590 IR, 565-615 IR & 650-710 IR), KP 685, LP 505 & LP 560
confocal_leuven_Olympus-fluoview-FV1000_cleaned

Olympus FluoVieuw FV1000

Location KU Leuven, campus Gasthuisberg, Building O&N1
6th floor, shared with the imaging core (CIC) at KULeuven
Type Olympus inverted
Light Source Laser; 405nm, 458nm, 488nm, 514nm, 561nm & 633nm
Properties designed for live imaging, motorized stage, CO2 and O2 incubator, heating stage and zero drift compensation
Objective lenses 4X UPLSAPO (0,16 dry), 20X UPLSAPO (0,75 dry), 40X UPLSAPO (0,90 dry) & 60X UPLSAPO (1,36 oil)
info

General Information

 

Booking Booking is available online for KU Leuven members
Storage For VIB members, a storage is offered