Confocal Microscopes – Ghent
Zeiss Spinning Disc
| Name: | Zeiss AxioObserver Spinning Disc (Yokogawa CSU-X1) |
| Application: | 3D live cell imaging, whole mount imaging |
| Type: | Invert confocal |
| Light Source: | 405, 488, 561, 639 (diode lasers) |
| Objectives: | EC Plan-Neofluar 10x/0.30 Ph 1 Plan- Apochromat 20x/0.80 LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC Plan-Apochromat 40x/1.40 Oil DIC UV-Vis-IR Plan-Apochromat 63x/1.40 Oil DIC |
| Detectors: | AxioCam MRm Photometrics Prime 95B |
| Filters: | DAPI (BP 445/50) HE GFP (FE01-520/35) YFP (BP 535/30) Texas Red (FE01-617/73) Cy5 (BP 690/50) DIC Analyzer for Transmitted Light |
| Room Location: | W281 |
Zeiss LSM 710
| Name: | ZeissLSM710 |
| Application: | High resolution confocal imaging; two-photon excitation for deep tissue and whole organ imaging; spectral unmixing |
| Type: | Upright confocal |
| Light Source: | Diode lasers: 561, 633 Argon laser: 458, 488, 514 MaiTai DeepSee multiphoton laser: range 680 – 1040 nm |
| Objectives: | Plan-Apochromat 5x/0.16 Plan-Apochromat 10x/0.45 W N-Achroplan 20x/0.5 Water Plan-Apochromat 20x/0.8 EC Plan-Neofluar 40x/0.75 IR-Achroplan 40x/0.80 Water Plan-Apochromat 63x/1.4 Oil DIC |
| Detectors: | Classical PMT (range 415-735 nm) Range detector 418-726nm 2 NND +2 BIG Detectors (LP538->LP490 SP485->BP665-610) |
| Filters: | Visible track: MBS 458, 458/514, 458/561, 488, 488/561, 488/561/633, MBS T80/R20 Invisible track: MBS -405, -445, 690+,760+, -405/760+, -445/760+, T80/R20 Extra filters NDD: LP510, LP580, Mirror/BP500-550, LP555/BP565-610 Extra filters BIG: BP525-560/BP650-705 |
| Room Location: | W285 |
Zeiss LSM 780
| Name: | ZeissLSM780 |
| Application: | High resolution confocal imaging; two-photon excitation for deep tissue imaging; live cell imaging; FLIM and FRET/FLIM; spectral unmixing |
| Type: | Invert confocal |
| Light Source: | Diode lasers: 405, 561, 633 Argon laser: 458, 488, 514 MaiTai DeepSee multiphoton laser: range 680 – 1040 nm |
| Objectives: | Plan-Apochromat 10x/0.45 Plan-Apochromat 20x/0.8 iLCI Plan-NeoFluar 25x/0.8 Imm Korr DIC Plan-Apochromat 40x/1.4 Oil DIC Plan-Apochromat 63x/1.4 Oil DIC iLCI Plan-NeoFluar 63x/1.3 Imm Korr DIC |
| Detectors: | Classical PMT (range 415-735 nm) QUASAR 32-channel GaAsP detector (range 412-692 nm) Cooled PMT (range 415-735 nm) |
| Filters: | Visible track: MBS 458, 458/514, 458/561, 488, 488/561, 488/633, 488/561/633, MBS T80/R20 Invisible track: MBS -405, 445, 690+, -405/760+, -445/760+, T80/R20 |
| Room Location: | W280 |
Zeiss LSM880 FastAiryScan
| Name: | Zeiss LSM880 Fast Airy |
| Application: | Super-resolution confocal imaging (dXY: 140 nm and dZ: 400 nm); fast and sensitive confocal imaging; regular confocal imaging; live cell imaging |
| Type: | Invert confocal |
| Light Source: | Diode lasers: 405, 561, 633 Argon laser: 458, 488, 514 |
| Objectives: | Plan-Apochromat 10X/0.45 iLCI Plan-Neofluar 25X/0.8 imm Korr DIC Plan-Apochromat 40X/1.4 oil DIC Plan-Apochromat 63X/1.4 oil LCI Plan-Neofluar 63X/1.3 imm Korr DIC alpha Plan-Apochromat 100X/1.46 oil DIC |
| Detectors: | Classical PMT (range 415-735 nm) Cooled PMT (range 415-735 nm) |
| Filters: | Main Beam Splitters: MBS 458, 458/514, 458/561, 488, 488/561, 488/561/633, T80/R20, -405, -445 Secondary Beam Splitters: SBS BP 420-460 + LP 500, LP 525, LP 570, LP 660, LP 460, SP 615 Airy Beam Splitters: BP 420-480 + BP 495-550, BP 555-620 + LP 645, BP 420-480 + LP 605, BP 465-505 + LP 525, BP 495-550 + LP 570, BP 570-620 + LP 645 |
| Room Location: | W284 |
Request for project intake
| For intakes of all LM projects, please contact Eef.Parthoens@irc.vib-ugent.be |