ConfocalConfocal Microscopes – Ghent

TCS_SP5_1_c89cf12293
IRC

Leica TCS SP5 AOBS

Location UGent-VIB Research Building FSVM
Room W276
Type Inverted
Light Source Laser; UV diode 405nm, Multi Ar: 458,488, 514 nm, HeNe 543 nm, HeNe 633
Properties FRAP, FRET
Objectives 10x HC PL Apo CS 0.4 Dry , 20x HC PL Apo 0.7 IMM (water, oil, glycerin),40x HCX PL Apo CS 1.25 oil, 63x HCX PL Apo 1.4 oil
Camera spectral photomultipliers DFC320 Color camera, 36 bit, 7Mpixels (non confocal)
Filters laser 1 prism + 3 slits make it possible to detect all emission signals from UV to IR
microscope_confocale
IRC

Zeiss Spinning Disk

Location UGent-VIB Research Building FSVM
Room W281
Type Observer.Z1
Light Source HXP 120C
Properties High resolution images, functional studies, and 3D time lapses
Objectives EC Plan-Neofluar 10X Ph 1 (0.30 dry), EC Plan-Neofluar 10X (0.30 dry M27), LD LCI Plan-Apochromat 25X (0.8 imm corr DIC M27), Plan-Apochromat 40X (1.40 oil DIC UV-Vis-IR M27), Plan-Apochromat 63X (1.40 oil DIC M27)
microscope_confocale

Zeiss LSM 780

Location UGent-VIB Research Building FSVM
Room W282
Type Observer.Z1
Light Source HXP 120C
Properties High resolution images, functional studies, and 3D time lapses
Objectives Plan-Apochromat 10X (0.45 M27), I LCI Plan-Neofluar 25X (0.8 imm Korr DIC M27), Plan-Apochromat 40X (1.4 oil DIC M27), Plan-Apochromat 63X (1.4 oil M27), LCI Plan-Neofluar 63X (1.3 imm Korr DIC M27), alpha Plan-Apochromat 100X (1.46 oil DIC M27). Filter for simultaneous imaging: BS 565, BP1 512/30, BP2 630/98
microscope_confocale

Zeiss TIRF Observer z.1

Location UGent-VIB Research Building FSVM
Room W283
Type Observer.Z1
Light Source HXP 120C
Properties superresolution in the evanescent field immediately above the coverslip, functional studies and time lapses (definite focus control)
Objectives 100x PlanApo NA: 1.46, oil immersion
Camera 2x EMCCD Hamamatsu ‘ImageMx2’ (simultaneous imaging is possible)
Filters 74HE CFP/YFP/Dsred
76 CFP/GFP/Dsred
77 HE GFP/mRFP/Alexa 633
74 HE GFP/mRFP
info

General Information

Data Storage VIB ONLY : Spinning Disk Time lapse: 70€/dataset | Spinning Disk: 27.5€/dataset | Confocal: 27.5€/dataset
Technical Support VIB : 50€/h