Superresolution Microscopes – Ghent
Zeiss TIRF Observer z.1
TIRF:
| Name: | Zeiss Observer Z.1 TIRF |
| Application: | TIRF microscopy (super-resolution imaging in the evanescent field immediately above the coverslip, mostly used for studying events at the plasma membrane with a z-resolution up to 80 nm); functional studies and live cell imaging with definite focus control |
| Type: | Invert widefield fluorescence; TIRF |
| Light Source: | 405, 488, 561, 639 (diode lasers) |
| Objectives: | 100x PlanApo NA: 1.46, oil immersion |
| Detectors: | 2 EMCCD Hamamatsu cameras ImageMx2 |
| Filters: | HE CFP/YFP/Dsred ((DBP 480/30 + 565/25 (HE), DFT 505 + 583 (HE), DBP 525/31 + 616/57 (HE)) CFP/GFP/Dsred ((TBP 406 + 489 + 561 (HE), TFT 427 + 503 + 578 (HE), TBP 459 + 525 + 608 (HE)) HE GFP/mRFP/Alexa 633 ((EX TBP 483+564+642; BS TFT 506+582+659; EM TBP 526+601+688) HE GFP/mRFP ((EX DBP 480/30+565/25; BS DFT 505+583; EM DBP 525/31+616/57)) |
| Room Location: | W282 |
Zeiss LSM880 FastAiryScan
| Name: | Zeiss LSM880 Fast Airy |
| Application: | Super-resolution confocal imaging (dXY: 140 nm and dZ: 400 nm); fast and sensitive confocal imaging; regular confocal imaging; live cell imaging |
| Type: | Invert confocal |
| Light Source: | Diode lasers: 405, 561, 633 Argon laser: 458, 488, 514 |
| Objectives: | Plan-Apochromat 10X/0.45 iLCI Plan-Neofluar 25X/0.8 imm Korr DIC Plan-Apochromat 40X/1.4 oil DIC Plan-Apochromat 63X/1.4 oil LCI Plan-Neofluar 63X/1.3 imm Korr DIC alpha Plan-Apochromat 100X/1.46 oil DIC |
| Detectors: | Classical PMT (range 415-735 nm) Cooled PMT (range 415-735 nm) |
| Filters: | Main Beam Splitters: MBS 458, 458/514, 458/561, 488, 488/561, 488/561/633, T80/R20, -405, -445 Secondary Beam Splitters: SBS BP 420-460 + LP 500, LP 525, LP 570, LP 660, LP 460, SP 615 Airy Beam Splitters: BP 420-480 + BP 495-550, BP 555-620 + LP 645, BP 420-480 + LP 605, BP 465-505 + LP 525, BP 495-550 + LP 570, BP 570-620 + LP 645 |
| Room Location: | W284 |